The experimental design was conducted in the soil culture trial to study the effect of two fungus(Penicillium oxalicum I1 and Aspergillus niger H1)on the soil dynamic changes of pH,available phosphorus,exchangeable Ca and Mg and available Fe,Mn,Cu,Zn.The results showed that when the two fungi were applicated,soil pH decreased significantly;soil available phosphorus,exchangeable Ca and Mg and available Fe,Cu,Zn increased significantly;available Mn decreased obviously.On the whole,the Penicillium oxalicum′s ability of increasing available soil nutrient elements was higher than Aspergillus niger′s.The result demonstrated that the contents of available soil nutrient elements were changed remarkably when Penicillium oxalicum was added.Compared with the control,the content of available phosphorous at 21th day increased 35.89%,the content of exchangeable magnesium,available Zn at 12th day increased 20.02% and 37.4% respectively;the content of available Fe at 15th day increased 43.0%;the content of exchangeable Ca and available Cu at 18th day increased 14.51% and 46.9%.However,the content of available Mn at 21th day decreased 32.8% in the treatment with Penicillium oxalicum.