The effect of organic manure and phosphate fertilizer on soil organic matter and humus content and composition was studied in a long term experiment.The results showed that:compared with the basic experiments soil,the soil organic matter content increased significantly by 8.4%~17.3% for the organic manure treatment and the combined application treatment of organic manure and phosphate fertilizer,and decreased significantly by 7.8%~10.7% for the phosphate fertilizer treatment during a 10 years period with fertilizer application at every other year.Compared with the P0M0 treatment,the organic matter content increased by 2.9%~6.2%,24.8%~29.8%,25.2%~35.1% respectively for phosphate fertilizer,organic manure application pattern and combined application of organic mannure and phosphate fertilizer.There was a significant positive correlation between the amount of soil humic carbon or humic acid carbon and soil organic matter content.Application of phosphate was good for the accumulation of soil fulvic acid,it could affect the proportion of soil humic acid and fulvic acid.