In order to determine the feasibility of SPAD value and hyperspectra data of different maize varieties to diagnose nitrogen nutrition status,it was studied that the variation of total nitrogen content，leaf spectral reflectance，SPAD values and the correlation of total nitrogen with leaf spectral reflectance and SPAD values in six maize varieties such as Zhongdan 909,Longping 206,Zhengdan 958,Jinsai 06-9,Nonghua 101 and Denghai 605 in Huabei region by sand culture with four nitrogen levels(0.04,0.4,2.0,4.0 mmol/L N).Results showed that,in the shooting stage，variation between nitrogen level was far greater than the varieties and the interaction between the two factors.The correlation between spectral reflectance at 550 nm and nitrogen content was significant negative(R=-0.808 8),the SPAD values and nitrogen content had a significant positive correlation(R=0.895 6).So it is feasible to observe maize nutrient with the hyperspectra data and SPAD value in Huabei area,and to diagnose nitrogen nutrition status,using SPAD value is more accurate than using the hyperspectra data.