The different type of fertilizer, irrigation quota, irrigation time, irrigation frequency were used in drip irrigation with fertilizer on rubber diminutive polytube budding. The result showed that: (1)Solid fertilizer was difficult to be diluted, the fertilizer damage symptoms would appear if the fertilizer wasnt uniformed in drip irrigation. Liquid fertilizer was easy to be diluted, wouldnt block the dropper, contained balanced nutrition, and was safe and reliable. (2)Two groups of drip irrigation with fertilizer system were screened out for rubber diminutive polytube budding. The first one was using liquid fertilizer with high frequency of fertilization. Every 3 days 45 mL farm biogas slurry fertilizer would be used in drip irrigation in 60 minutes for each strain of diminutive polytube budding. The second one was using less solid fertilizer with suitable application frequency. Every 6 days 0.5 g conventional compound fertilizer was dissolved and diluted and applied to each strain of diminutive polytube budding with drip irrigation in 60 minutes. (3)Applied farm biogas slurry fertilizer with drip irrigation had the best effect to the net growth of scionportion, root performance and leaves nutrient content.