元素分析仪和全自动凯氏定氮仪测定土壤全氮之比较
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中央级公益性科研院所专项资金资助项目(IARRP-2014-39)。


Comparison of element analyzer (Dumas Combustion) and automated Kjeldahl analyzer( Kjeldahl methods) for determining total nitrogen content in soils
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    摘要:

    土壤全氮含量反映土壤中氮循环的状况,是评价土壤肥力与土壤资源的一项重要指标。选择黑土和潮土,分别利用元素分析仪(杜马斯催化燃烧法)和全自动凯氏定氮仪(凯氏消煮蒸馏法)测定土壤全氮含量,以揭示两种仪器测定结果的可比性,每个样品每种方法重复测定5次。结果表明:元素分析仪和全自动凯氏定氮仪测定的黑土全氮含量(平均值±标准偏差)分别为(1.37±0.01)g/kg和(1.36±0.01)g/kg,潮土全氮含量分别为(0.75±0.02)g/kg和(0.78±0.03)g/kg,t检测表明,两种仪器测定结果之间无显著性差异。两种仪器土壤全氮测定结果精确度均较高,稳定性较好,相对标准偏差均小于5%。本研究结果可为采用仪器方法测定土壤全氮含量及评价土壤氮库变化提供参考。

    Abstract:

    Nitrogen (N) is one of the most important elements for plant nutrition.It is necessary to apply reliable methods for N determination in soils.Among the different methods of total N determination in soils,the Kjeldahl method (wet oxidation) and the elemental analyzer (Dumas dry combustion method) are more frequently used.In this study,black soil was sampled from Heilongjiang provinces in Northeast China and calcareous fluvoaquic soil sample was from Shandong provinces in North China Plain.The objective of this study was to compare soil total N contents determined by a CNS elemental analyzer (vario PYRO cube,Elementar Analysensysteme GmbH) and an automated Kjeldahl N determination instrument (BCHI Labortechnik AG).The results showed that total N contents of black soil samples measured by the elemental analyzer and the automated microKjeldahl method were(1.37±0.01)g/kg and(1.36±0.01)g/kg(mean±SD),respectively.The N contents of fluvoaquic soil samples were(0.75±0.02)g/kg through the elemental analyzer method and(0.78±0.03)g/kg by the automated Kjeldahl method.The t test found there was no significant difference of soil N measured by two kinds of instruments.Both methods for soil N measurement had a high precision with relative standard deviation (RSD) less than 5%.

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李桂花,叶小兰,吕子古,杨森,温云杰,刘云霞,汪洪*.元素分析仪和全自动凯氏定氮仪测定土壤全氮之比较[J].中国土壤与肥料,2015,(3):111-115.

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  • 收稿日期:2014-06-08
  • 最后修改日期:2014-07-04
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  • 在线发布日期: 2015-06-24
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