脲酶抑制剂氢醌对土壤脲酶动力学行为的调控效应
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国家自然青年科学基金(41301253);辽宁省农业领域青年科技创新人才培养计划(2014018);辽宁省博士启动基金(20121145)


Regulation effects on soil urease kinetics as affected by urease inhibitor hydroquinone
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    摘要:

    研究了不同温度条件下脲酶抑制剂氢醌(HQ)对东北3种典型土壤(白浆土、棕壤、褐土)脲酶动力学参数的影响。结果表明,土壤类型、培养时间、培养温度及其相互作用均显著影响土壤脲酶动力学参数。与对照相比,加入HQ使土壤脲酶米氏常数(Km)增加,最大反应速率(Vmax)降低,表明HQ对土壤脲酶的作用机理属于混合型抑制。与白浆土相比,棕壤和褐土脲酶动力学参数受HQ的影响程度较大,表明高肥力土壤生物学活性较稳定。随着培养时间延长,土壤脲酶Km降低,Vmax和Vmax/Km增加。随着温度升高,土壤脲酶Km和Vmax增加,Vamx/Km无规律性变化。相关性分析表明,土壤脲酶动力学参数Km、Vmax和Vmax/Km与pH值、有机质、全氮、碱解氮和质地组成之间存在显著相关关系。

    Abstract:

    The effect of urease inhibitor quinhydrone(HQ)on kinetic parameters of urease in three typical soils(iealbic soil,brown soil,cinnamon soil)in Northeast China under different temperature conditions.The results showed that,soil type,incubation time,incubation temperature and their interactions significantly influenced soil urease kinetics.Compared with control,Km increase and Vmax decrease in the soils with HQ and it showed HQ was mixed inhibition to soil urease.Compared with albic soil,in brown soil and cinnamon soil the influence on kinetic parameters of soil urease as affected by HQ was greater,which indicated that high fertility soils could have stable biological characteristics.With incubation time increasing,Km decreased,but Vmax and Vmax/Km increased.With incubation temperature increasing,Km and Vmax increased,Vmax/Km changed irregularly.Statistical analysis indicated that,Km,Vmax and Vmax/Km of soil urease had significantly correlations with pH,organic matter,total nitrogen,alkalihydrolyzed nitrogen and texture structure.

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隽英华,陈振华,张玉兰,张丽莉,陈利军.脲酶抑制剂氢醌对土壤脲酶动力学行为的调控效应[J].中国土壤与肥料,2015,(4):53-58.

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  • 收稿日期:2014-05-27
  • 最后修改日期:2014-09-15
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  • 在线发布日期: 2015-08-18
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