Two-year field plot experiment was carried out to study the photosynthetic characteristics and yield and quality when biogas manure was jointly applied with chemical fertilizer in Northeast spring maize production of China.Under the control of total input of nitrogen application,the effects of different proportion of biogas manure and the chemical fertilizer on maize photosynthesis,yield and quality were studied.The results showed that suitable proportion of biogas manure and chemical fertilizer in soil could improve maize leaf photosynthetic characteristics,and increase leaf chlorophyll content(SPAD value),transformation of primary energy efficiency of PSII optical system(Fv/Fm),the potential activity of PSII(Fv/F0),photosynthetic rate(Pn),transpiration rate(Tr),stomatal conductance(Cond)and photosynthetic rate.The treatment of A1(2 000 g biogas manure+500 g chemical fertilizer)showed the best performance on grain yield,which was as high as 11 344.1 kg/hm2.The trend of yield among the treatments showed as follows:A1＞A2＞A3＞A4＞CK.At the same time,the quality index of crude protein,crude fat and crude corn starch grain was obviously improved.The water content of grain decreased,which was beneficial to the harvest period of spring maize in Northeast China.