氮肥对镉胁迫下结缕草光合和叶绿素荧光特性的影响
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国家自然科学基金项目(31470398;31270369)


Effects of nitrogen fertilizer on photosynthetic and fluorescent characteristics of Zoysia japonica under cadmium stress
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    摘要:

    采用盆栽试验,研究了不同氮肥用量[0、200、300 和 400 mg/kg CO(NH2)2]对Cd胁迫(50 mg/kg)下结缕草光合及叶绿素荧光特性的影响。结果表明:不同浓度的氮肥均能使Cd胁迫下结缕草叶片叶绿素含量、净光合速率(Pn)、气孔导度(Gs)、胞间二氧化碳浓度(Ci)、蒸腾速率(Tr)、光化学猝灭系数(qP)、叶片PSⅡ最大光量子产量(Fv/ Fm)及表观光电子传递效率(ETR)有所提高,而非光化学猝灭系数(NPQ)有所降低,说明氮肥对Cd污染具有一定的缓释作用。从结缕草叶片叶绿素(叶绿素a、叶绿素b、叶绿素a + b)含量、气孔导度、胞间二氧化碳浓度、叶片PSⅡ最大光量子产量及表观光电子传递效率指标来看,以氮肥浓度300 mg/kg缓解作用最大,200 mg/kg次之,400 mg/kg最弱;相对于叶片净光合速率、蒸腾速率、水分利用率(WUE)、非光化学猝灭系数来说,以氮肥浓度300 mg/kg缓解作用最为明显,400 mg/kg次之,200 mg/kg最弱;不同施肥浓度及镉胁迫对结缕草叶片光化学猝灭系数基本无影响(P>0.05)。通过本研究得出:施用氮肥对Cd胁迫下结缕草具有一定的缓解作用,当氮肥浓度为300 mg/kg时缓解效果最为显著。

    Abstract:

    The method of pot culture was used to investigate the proper application rate of the nitrogen fertilizer during the period of under cadmium stress(50 mg/kg)plant growth.The study was carried out to examine the effects of different dosages nitrogen fertilizer[0,200,300 and 400 mg/kg CO(NH2)2]on photosynthesis and fluorescence characteristics of Zoysia japonica under cadmium stress.The results showed that different dosages nitrogen fertilizer enhanced chlorophyll contents,net photosynthetic rate,stomatal conduction,intercellular CO2 concentration,transpiration rate,photochemical quenching,maximum photo quantum yield,and the electron transfer rate and decreased nonphotochemical quenching(NPQ)under Cd stress,which demonstated that N fertilizer could release the effect of Cd stress on Zoysia japonica.For the effects of different nitrogen concentration on chlorophyll content of Zoysia(Chl a,Chl b,Chl a + b),stomatal conductance(Gs),intercellular carbon dioxide concentration(Ci),leaf maximum quantum yield of PSⅡ(Fv / Fm)and the electron transfer rate(ETR),it showed that treatment with 300 mg/kg nitrogen was the best,then 200 and 400 mg/kg.For the effects of different nitrogen concentration on net photosynthetic rate(Pn),transpiration rate(Tr),water use efficiency(WUE)and nonphotochemical quenching(NPQ),the result was that treatment with 300 mg/kg nitrogen was better than that of 400 and 200 mg/kg.However photochemical quenching(qP)was not significantly affected(P>005)by nitrogen concentration.It was concluded that 300 mg/kg nitrogen concentration had the greatest effect on Zoysia japonica under cadmium stress in this study.

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魏花朵,李悦,陈忠林,徐苏男,于宁,张利红*.氮肥对镉胁迫下结缕草光合和叶绿素荧光特性的影响[J].中国土壤与肥料,2015,(4):88-92.

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  • 收稿日期:2014-07-13
  • 最后修改日期:2014-10-18
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  • 在线发布日期: 2015-08-18
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