To explore the effect of green manure application on agronomic characters of tobacco and decrease nematodes harm to tobacco replant,a field plot experiment,with ten treatments which were assembled by two levels of chemical fertilizer and five levels of green manure,was conducted in red soil in Yunnan.Results showed that leaf size,plant height and stem diameter of tobacco were significantly or extreme significantly positive correlated to the amount of green manure application.With the increasing of green manure application amount,the quantity of parasitic nematodes and nonparasitic nematodes tended to increase in red soil at the end of tobacco harvesting,but the degree of nematodes harm to tobacco root was extremely significantly negative correlated to the amount of green manure application.In summary,green manure application could promote tobacco growth and resist parasitic nematodes harm by nutrients supply improvement.In agricultural practice,15 000 kg/hm2 of green manure was usually applied for achieving proper and high quality fluecured tobacco product.