In this work，cell growth and sporulation of Bacillus subtilis DK36 for microorganism composting preparations were investigated using degossypolized cottonseed protein as nitrogen source.The results showed that the cells grew very well when degossypolized cottonseed protein was used as nitrogen source，and it could replace soybean meal and even yeast extract.The cells of Bsubtilis DK36 did not sporulate when glucose was used as carbon source.The cells produced a great amount of spores when corn meal was used as carbon source.The treatment with corn meal had the highest spore yield and sporulation efficiency，which were 2.7×109CFU/mL and 93.4%，respectively.Supplement of Ca2+，Mg2+，Mn2+ and K+ improved significantly spore yield and sporulation of Bsubtilis DK36.K+ had a synergism with Ca2+，Mg2+ and Mn2+ in stimulation of the cell growth and sporulation.KH2PO4 and MgSO4 had the best synergism effect，and its spore yield and sporulation efficiency were 3.2×109CFU/mL and 98.4% respectively.However，degossypolized cottonseed protein made sporulation of the cells very slowly，which greatly reduced the production efficiency.