The effects of different nitrogen(N)levels(0,2,4,8,12,16,20 mmol/L)on the physiological characteristics of Asparagus Cochinchinensis(Lour.)Merr were studied using sand culture method.The results showed that stem growth was positively correlated with N levels.The rhizome weight and chlorophyll content increased at firstly,and then decreased with the increasing of N supply,and reached the maximum values at the N level of 16 mmol/L.The amino acids and soluble sugar showed same change trend as the rhizome weight and chlorophyll content,which reached a higher level at 12～16 mmol/L.As a whole,N nutrient had a significant effect on the growth and quality of Asparagus Cochinchinensis(Lour.)Merr.High nitrogen cultivation(12~16 mmol/L)was suitable for eating purpose,and appropriate lower nitrogen level(2～8 mmol/L)was suitable for being used as medicine.