Effects of the combined application of molybdenum and nitrogen on the molybdenum accumulation and distribution in fluecured tobacco were investigated at different growing stage with pot experiment.The results showed that the molybdenum concentration and accumulation in tobacco increased with the increasing of molybdenum application rate at rosette stage,growing stage,topping stage and maturity stage.There was a negative interaction effect between the nitrogen and molybdenum application.The molybdenum concentration and accumulation in tobacco decreased with the increasing of nitrogen application rate at different growing stages.The accumulation rate in tobacco increased rapidly from the growing stage to topping stage and peaked at the 62th day after transplanting.Molybdenum application increased the proportion of molybdenum distribution in the upper leaves and decreased the proportion in the lower leaves under higher nitrogen levels (N2,N3).