土壤中钙、氮含量对番茄枯萎病抗性的影响
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国家自然科学基金(41171192)


Effect of different available calcium and nitrogen in soil on effectiveness of disease resistance to blight of tomato
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    摘要:

    采用土培的方法,研究土壤中钙、氮含量对番茄接种枯萎病病原菌后其体内有效钙含量、干物质重及番茄抗病指标变化的影响。结果表明,接种病原菌后各处理番茄病情指数、叶片组织苯丙氨酸解氨酶(PAL)、多酚氧化酶(PPO)、过氧化物酶(POD)和过氧化氢酶(CAT)活性、丙二醛(MDA)和细胞膜透性均显著提高,以土壤有效钙为241.36 mg·kg-1、碱解氮为553.63 mg·kg-1条件下番茄有效钙含量及干物质重较高,病情指数较低、4种防御酶活性增加幅度较大且峰值较高,丙二醛和细胞膜透性较低,氮含量随土壤碱解氮的增加而增加。因此,在土壤碱解氮过多处理中施入适量钙肥可以缓解其对番茄抗枯萎病的抑制作用,提高钙素有效性,使番茄具有较高抗枯萎病能力。

    Abstract:

    The soil culture were carried out to study effect of available calcium and nitrogen nutrition on the effective calcium content,dry weight,and the changes of the diseaseresistant indexes in the tomato after inoculation of pathogenic bacteria.The results showed that disease indexes,leaf tissues of phenylalanine ammonia lyase(PAL),polyphenol oxidase(PPO),peroxidase(POD),catalase(CAT)activity,malondialdehyde(MDA)and cell membrane permeability were significantly increased through the inoculation,especially in the condition that calcium was 241.36 mg·kg-1 and available nitrogen was 553.63 mg·kg-1 in soil,of which the effective calcium content and dry weight were higher,disease index was lower,four defensive enzymes activity increased quickly and peak were higher,MDA and cell membrane permeability were lower through inoculation in treatments.Nitrogen content was increased with the increase of soil available nitrogen.In summary,the right amount of calcium relieved the inhibition to resistance ability of the tomato blight and improved the effectiveness of calcium in the soil with high nitrogen content and improved the diseaseresistant ability to tomato blight.

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于威,依艳丽*,杨蕾.土壤中钙、氮含量对番茄枯萎病抗性的影响[J].中国土壤与肥料,2016,(1):134-140.

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  • 收稿日期:2014-12-09
  • 最后修改日期:2015-01-14
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  • 在线发布日期: 2016-02-05
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