Calcium is the most important essential element on the growth of cucumber.The cucumber seedlings were grown in substrate culture with pots,the Netherlands Gardening formula was used as the control,but the calcium content was changed while the other nutrient concentration remained constant to determine the most suitable local nutrient solution formula.Yield and quality(protein,Vc,nitrates,organic acid and soluble sugar) of cucumber were analyzed.The result showed that the increment of 1.2 Calcium was the optimized nutrient solution with best yield and qulity,plant height and stem diameter.Four different concentration gradients of nutrient solution treatment were designed based on the results of the first research stage(EC=1.5,2.5,3.5,4.5 mS·cm-1,respectively).The plant heights of EC 1.5 mS·cm-1 treatment and 3.5 mS·cm-1 treatment were higher than those of other treatments in early growth and blossom period of the cucumbers.The ratio of plant height/stem diameter for 3.5 mS·cm-1 was smaller than others in the vegetative growth stage.In addition,the cucumber yield and the qualities of the 3.5 mS·cm-1 treatment which were analyzed through the weighted average method were better than the other treatments.It indicated that 393.176 mg·L-1 was the suitable level of calcium of cucumber with the best growth and the highest yield under the experiment of production in the substrate.