A pot experiment was carried out to investigate the effects of inoculation of different arbuscular mycorrhizal（AM）fungi［Funneliformis mosseae （BGCNM03D），Claroideoglomus etunicatum（BGCNM01B），Rhizophagus intraradices（BJ09）］on growth，nitrogen uptake and expression of 5 nitrate transporter（NRT NAR）and 2 ammonium transporter（AMT）genes in roots of wheat under low N（no N application）and high N（N 0.2 g·kg1 soil）treatments.The results indicated that wheat roots were colonized by all the three AM fungi，and the fungus R.intraradices had the highest colonization rate in both N treatments.The inoculation of Rintraradices and Cetunicatum significantly increased shoot biomass or shoot N accumulation compared with the nonmycorrhizal control.The transcription levels of all the NRT and AMT genes in roots of wheat were downregulated by inoculating the AM fungi in both N treatments，and different AM fungi exhibited different abilities to regulated the expression of the NRT，NAR and AMT genes.