This experiment were studied on the basis of the soil removal of longterm sitespecific experiment field,using fluorescence spectroscopy method to analyze the fluorescent structure of HA before and after the removal.This study revealed the influence effect of the removal on fluorescent structure of HA under the removal of black soil and puting forward a feasibility basis for removal of longterm sitespecific experiment field.From the fluorescence spectrum of HA,it showed that the characteristic peak wavelength had different degree of red shift phenomenon in the first year after soil removal,with a slight aging phenomenon and the degree of condensation and more aromaticity.These aging phenomena were more obvious in manure and chemical treatment and manurel treatments.These phenomena eased in the second year after removal and had no obvious regularity between two different layers.The simple structure of aromatic compounds of HA molecules were out of the disturbance of soil removal.