To study the effect of continuous cropping on sorghum growth, yield, the variation of soil enzyme activity and microorganism, the cornsorghum rotation was employed as control. The results showed that a significant inhibition on growth and yield was observed when the sorghum was continuous cropping for 3 years. The sorghum continuous cropped for 3 years significantly decreased the plant height, stem diameter, leaf area and biomass, the difference was more obvious in 4 years. Sorghum continuous cropping for 4 years also had a severe influence on the root growth. Particularly, in the depth between 0~40 cm, compared with the index of cornsorghum rotation, the root biomass, root length, root surface area and root volume collected for sorghum continuous cropping were 61.5%, 84.4%, 73.8% respectively of those of the cornsorghum rotation. Meanwhile, The number of fungi in continious cropping soil was 1.9 times and 1.3 times of that in rotation at jointing stage, and at the filling stage. There was no apparent pattern for the change of bacteria and actinomyces with continuous cropping for 4 years. Besides, the catalase activities and invertase activities of continuous cropping for 4 years was increased by 14.7％, 17.2％ than that of the rotation cropping. It included that the sorghum continuous cropping restrained the growth of sorghum, and significantly impacted the soil microflora and enzyme activity.