In order to study the effect of increasing application of phosphorus bacterial fertilizer on soil phosphorus supply and phosphorus validity,the effects of continuous application of P bacterial fertilizer on alkaline phosphatase activity activity and Hedley phosphorus forms were studied by field experiments in 5 year reclamation soil.The results showed that：Alkaline phosphatase activity in the treatment with application of inorganic fertilizer+organic fertilizer+phosphorus bacterial fertilizer was the highest,which was 30.65 μg/(g·h)and increased by 83.86% than that of the control.Compared to the third year of reclamation soil,the contents of different forms of Hedley phosphorus in soil were increased in different degrees,the H2OPo content of inorganic fertilizer+organic fertilizer+phosphorus bacterial fertilizer treatment was the highest,increased by 93.90% than the control.NaOHPi,HClPi,HClPo contents of inorganic fertilizer + phosphorus bacterial fertilizer treatment were the largest,increased by 194.2%,61.87%,105.8%,respectively.The effect of inorganic fertilizer on phosphorus content in compound fertilizer residue was the greatest,which was increased by 22.87% than that of CK.The contents of H2OPi、NaHCO3Po of organic fertilizer treatment were the highest,which were increased by 129.2%,85.89% than those of the control.NaHCO3Pi,NaOHPo contents of organic fertilizer phosphorus bacterial fertilizer treatment were the maximum,increased by 176.9%,114.4%,respectively.It was concluded that the application of phosphorus bacterial fertilizer treatment had the better effect to the content of H2OPo，NaHCO3Pi，NaOHPi，NaOHPo，HClPi、HClPo in reclaimed soil.H2OPi,H2OPo,NaHCO3Pi,NaHCO3Po,NaOHPi,NaOHPo,HClPi were significantly related with alkaline phosphatase.Phosphate bacterial fertilizer enhanced the reclaimed soil alkaline phosphatase activity to some extent,influenced soil content of different forms of phosphorus in Hedley grade of phosphorus,and enhanced the effectiveness of phosphorus.