A long term(threeyears)experiment was conducted to investigate the effect of partly replacing(20 %)chemical fertilizer by two different organic fertilizers(refined organic fertilizer and bioorganic fertilizer)on soil organic carbon and the quality of fluecured tobacco.The results indicated that:1)In the treatment with bioorganic fertilizer(BIO)replacing 20 % inorganic fertilizer,tobacco production value and high class tobacco ratio were increased by 24.99% and 27.27% comparing with treatment CF which followed conventional fertilization;and by 1868% and 24.92% comparing with refined organic fertilizer(OF)treated treatment.2)Compared with treatment of CF,the contents of total organic carbon(TOC),labile organic carbon(LOC)and carbon pool management index(CPMI)were increased by 15.34%,25.06% and 25.51% respectively in treatment OF;in the treatment of BIO,the contents of TOC,LOC and CPMI improved 20.00%,36.95% and 41.75% respectively,comparing with CF;Treatment with BIO showed better performance than OF which was proved by the increasing LOC content and CPMI 3)LOC and CPMI were significantly positive related with tobacco production value,average price and proportion of high class tobacco leaves.It was concluded that according to local soil fertility,using organic fertilizer replaced 20% chemical fertilizer,especially BIO,was advantage to the accumulation of soil organic carbon and increasing of LOC content and CPMI,improving soil fertility,increasing tobacco yield and quality.