To investigate the effect of different selenium application methods and application rate on the absorption ,distribution and accumulation of selenium in grape，the Kyoho grape cultured(grafted to beta rootstock)in open cultivation was used as test material.The results were shown as follows:(1)After the application of 0.01% amino acid selenium by foliage spraying and amino acid selenium(0.3 g/plant)into soil at fullbloom stage， the selenium contents in leaves，leafstalks，berry flesh and single berry increased at first and then decreased from flowering to fruit ripening .(2)Compared with the control，the orders of selenium accumulation changed after being treated with selenium，the orders of selenium by foliage spraying were leaf>berry flesh>leafstalks，the orders of the application of selenium into soil were berry flesh> leaf>leafstalks.(3)The distribution and accumulation of selenium in grape were affected by different application methods.The orders of selenium accumulation in the fruit were seed> berry flesh>skin by the application of selenium into soil，and skin> berry flesh> seed by foliage spraying with selenium.(4)The application of amino acid selenium by foliage spraying and into soil at fullbloom stage achieved the best results.The selenium contents in grape berries increased with the increase of exogenous selenium doses.