In order to elucidate the effect of biodegradation process of the straw seedling containers on enzyme activities and microflora of soil and container residues，a soilburied experiment were carried out with two types of straw containers，which were named as starch adhesive/straw seedling containers(Modified starch glue container，MSGC)and soybean adhesive/straw seedling containers(Modified Bean glue container，MBGC)，respectively.In this experiment，two treatments were buried into soil with platelets cut from straw container walls and the control(CK)was the ones without any platelets.The experiment lasted 45 days and enzyme activity and the number of microbes and microbial community diversity of soil and platelets were determined by the conventional analysis and BiologEco methods.The results showed that the enzyme activities of soil and platelets tended to rise in general.There was no significant difference of amylase activities of the platelets between MSGC and MBGC and cellulose enzyme activity of MSGC was significantly higher than that of MBGC in the early period but not significantly different in the later period.Nevertheless，protease activity of MBGC had always been significantly higher than that of MSGC in the whole process.Straw container promoted activity of soil urease and phosphatase，which of MSGC were higher compared to MBGC.BiologEco analysis showed that straw container significantly increased utilization of various carbon sources of soil microbes，and the value of soil AWCD increased in the beginning and decreased afterwards.The difference between MBGC and MSGC was that soil AWCD value of the latter declined significantly faster than that of the former.There was significant difference of microbial functional diversity in the early between two containers and the gap narrowed soon afterwards.Compared with MSGC，MBGC had advantage of soil microorganisms utilization activities of amino acids，polymers，and amine，which mainly related to the different container adhesives.The results of this study provided a theoretical basis for field application and evaluation of its ecological effect of straw seedling containers.