The effect of fertilizer combination on the soil enzyme activity was studied in spring maize farmland.The field location planted spring maize for 4 years.The soil enzyme activity was analyzed in the different growth period.The differences of dynamic changes of enzyme activity were revealed using the cluster discriminant analysis and the principal component analysis.The results showed that fertilization significantly increased the activities of sucrase,urease and phosphatase,and mainly affected the enzyme activity of 10~20 cm soil layer.The soil enzyme activity reached the maximum in the powder period with organic fertilizer application and in the big trumpet period with inorganic fertilizer application.With the increasing of soil depth,the activities of sucrase,urease and phosphatase decreased.Catalase activity reached the minimum in 10～20 cm soil layer.In various fertilization treatments,with the increasing of nitrogen content,the catalase enzyme activity decreased,and the sucrose and urease activity increased.The urease and phosphatase activity increased with more phosphate application.The catalase enzyme activity increased,and the sucrose and urease activity decreased when organic fertilizer was increased.While with only nitrogen fertilizer,urease and phosphatase activities reduced.Organic and inorganic combined fertilizers extended the high value duration of soil enzyme activity during the spring maizes growth period.The activity of sucrase,urease,catalase and phosphatase was influenced respectively by total potassium,total nitrogen,organic matter and total nitrogen,total phosphorus and organic matter content.Fertilization affected soil enzyme activity by changing soil elements content and supply ability.