A field experiment was conducted to determine the effects of single N application and split N application as tiller fertilization and earing fertilization on rice yield and composition,N fertilizer use efficiency,as well as tiller dynamic,leaf areas of high photosynthetic efficiency and SPAD value of machinetransplanted rice,which were closely related to the yield and N fertilizer use efficiency in the mode of rice transplanting with machine.The results showed split N application as tiller fertilization had little effect on yield development.However,split N application as earing fertilization increased rice yield by 6.1%～6.5%,and enhanced N fertilizer use efficiency by 10.0%～11.6%.The main reasons were split N applications as tiller fertilization had no effect on tiller dynamic and leaf age.But,split N applications as earing fertilization increased leaves’ areas of top first leaf and top second leaf by 10.1%～13.7% and 32.1%～39.9%,respectively,which reached significant levels.Meanwhile,chlorophyll degradation rate within 20 days after flower was retarded under treatment of split N applications as earing fertilization,which laid a good foundation for photosynthetic material accumulation during late growth stage,and then led to 5.1%～6.1% of increase in spike rate,and greatly improved the filled grain number.In sum,single N application of tiller fertilization and split N applications of earing fertilization on rice transplanting with machine increased yield,raised N fertilizer use efficiency and slowed down labor shortage.