With the suncured tobacco variety(Dayehuang),a field experiment was conducted to study the variation of soil nutrients in different growing periods and potassium concentration and accumulation in tobacco leaves.The relationship between soil nutrients concentration and potassium in tobacco leaves was analyzed in order to identify the best potassium fertilizer combination that could improve the accumulation of potassium and find the most important soil nutrients factors that affected the potassium accumulation.Results showed that the treatment T4 which applied biological potassium fertilizer combined with K2SO4 was the best.It had the highest dry matter accumulation,potassium concentration and potassium accumulation,which was respectively 143.5 g/plant,13.39 mg/g and 1.92 g/plant.Available sulfur mainly affected potassium concentration in the early growth stage.Available potassium concentration had a significantly or very significantly positive relation with potassium accumulation of tobacco leaves,and improved the potassium concentration of tobacco leaves in middle and later stages.