In this study，the effect of two probiotic (Bacillus megaterium and Bacillus amyloliquefaciens) combined with mineral fertilizer and vermicompost on soil properties，spinach yield and quality was investigated.The results showed that，under the same nutrient level，vermicompost significantly improved the content of soil available phosphorus and pH value，reduced the content of available potassium，ammonium nitrogen and EC value，increased the activities of favorable soil urease，protease and catalase，effectively improved the quality of spinach.Compared with the single application of vermicompost，probiotics with vermicompost fertilizer not only significantly increased soil nitrate content and invertase activity，but also increased the soil EC value，the spinach soluble sugar，protein and vitamin C content.Bacillus amyloliquefaciens combined with vermicompost also increased the yield of spinach.This improving efficiency was better than chemical fertilizers combined with probiotics.By comprehensive comparison of effects of the two kinds of probiotics and vermicompost，vermicompost with applied solution of Bacillus amyloliquefaciens had the best effect on soil properties improvement and vegetable yield and quality.