The objective of this field stimulation experiment was to study the effect of relative position of dripper and spot chicken manure on the spatial distribution of soil enzyme activities,in order to provide a theoretical basis and scientific guidance for field fertilization and irrigation.The experiment included two kinds of water position,ie0 cm and 30 cm away from the fertilizer application point.Manure application method was spot application.There were three treatments,CK-no fertilizer,CM0-water position and chicken manure in the same place,CM30-water position was 30 cm away from the fertilizer application point.Urease,invertase and alkaline phosphatase activities 10 cm,20 cm and 30 cm from the fertilizer position in horizontal direction and 60 cm in vertical direction from ground surface were investigated.The results showed that dripper and spot chicken manure application remarkably increased urease,invertase and alkaline phosphatase activities,especially in 0～20 cm soil layer,which arrived the maximum after 5 to 15 days and then decreased gradually.In soil profiles in control treatment and CM0 treatment,the enzyme activities were the highest at horizontal distances of 10 cm from the fertilizer application point and then decreased radially from the dripper.In the CM30 treatment,urease and invertase activities were the highest in 0~20 cm soil layer and at horizontal distances of 30 cm from the fertilizer application point after 5 days,and distributions of soil enzyme activities decreased with the distance from the dripper in soil profiles.With the incubation time prolonging,urease activity was the highest at horizontal 10 cm,and decreased from the fertilizer application point in soil profiles.Invertase activities had no the similar trend in the process,while alkaline phosphatase activity was higher at horizontal 10 cm and 30 cm than that at the horizontal 20 cm.In 20～40 cm soil layer,urease activity gradually decreased from fertilizer point after 25 days.Alkaline phosphatase activity was the highest at horizontal 30 cm with time.In conclusion,in agricultural practice water and fertilizer in the same position helped to improve activities of urease,invertase and alkaline phosphatase,enzyme activities were the highest at 10 cm from the fertilizer application point in topsoil.