In order to understand the effect of longterm fertilization on dynamics of crop yield,nitrogen use efficiency,five treatments including NPK,M(manure),0.5MNPK(1/2M+1/2NPK),MNPK and CK(no fertilizer)were carried out based on 19 years' longterm fertilizer experiment in paddy soil from yellow field in Guizhou province.The results showed that longterm application of chemical fertilizer or organic fertilizer significantly increased crop yield,the crop yield of NPK,M,0.5MNPK,MNPK treatments were increased by 56.2%,57.8%,65.0%,74.0%,respectively,compared with CK.The partial factor productivity of applied N(PFPN),inner efficiency of applied N(IEN),recovery efficiency of applied N(REN),accumulated recovery efficiency of applied N(AREN)of different fertilization treatments were 10.9～20.7 kg·kg-1,44.7～53.8 kg·kg-1,13.6%～19.6%,12.0%～16.9%,respectively.0.5MNPK treatment had the highest N use efficiency and MNPK had the lowest,which indicated that high N application rate resulted in lower N use efficiency.The different type of N use efficiency of NPK treatment were unchanged or decreased with time,while the N use efficiency in treatments with manure were significantly increased with time,especially the ARE was significantly increased with time.Longterm application of manure,especially manure combined with chemical fertilizers,had stable crop yield and the nitrogen fertilizer utilization rate increased steadily.Therefore,reasonable application of chemical fertilizer coupled with organic manure is an effective measure to improve crop yield,fertilizer use efficiency,and could reduce the amount of N fertilizer in the perennial high amount of paddy soil from yellow field.