In this study,the cow manure vermicompost,peat,perlite,and vermiculite were mixed with different proportions (the volume ratio of vermicompost,peat,perlite,and vermiculite in substrate a,substrate b,substrate c were 0∶4∶1∶1,2∶2∶1∶1,4∶0∶1∶1,separately),to select the best growth substrate for strawberry through the analysis of strawberry growth,yield and quality.The results showed that the substrates were general suitable for strawberry growth.Generally,the soluble sugar content,sugaracid ratio,soluble solids content,ascorbic acid content,soluble protein content,anthocyanins content of strawberry fruits in substrate b were higher than that in substrate a and substrate c.In addition,the harvesting time of strawberry fruit in substrate b was 5 ~ 15 days earlier than that in substrate a and substrate c.So the substrate b can be recommended for the factory production of strawberry growth.