Various phosphatesolubilizing bacterial strains were isolated from the long term planting of vegetable greenhouse rhizosphere in Huangpi district of wuhan city. After many times of screening and purification,a strain P1 with efficient phosphatesolubilizing capability was obtained. According to physiological and biochemical characteristics and 16S rDNA sequence,the strain of P1 was identified as belonging to Ensifer. The effect of different fermentation conditions on the phosphatesolubilizing capacity of P1 strain was studied. Under the optimal conditions in which the fermentation time was 7 days,initial pH was 8 and inoculum size was 2%,P1 dissolved 443.11 mg/L tricalcium phosphate. The study aslo found that the capacity of the P1 was closely negative correlated to the pH of medium.