盐胁迫环境下接种根际促生细菌对碱蓬根际土壤微环境特征的影响
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国家自然科学基金项目(31570614);山东省科技发展计划项目(2013GXH21007);山东省农业重大应用技术创新“耐盐根际促生细菌在黄河三角洲重盐碱地生态修复中的应用技术研究”项目。


Effects of inoculating plant growthpromoting rhizobacteria on the microenvironmental characteristics of the rhizosphere soil of Suaeda glauce Bge under salt stress
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    摘要:

    为探讨不同盐胁迫生境中接种根际促生细菌(PGPR)对碱蓬根际土壤微环境特征的作用效果,通过盆栽试验,研究了不同盐胁迫(轻度、中度、重度)及其接种蜡样芽孢杆菌(PGPR)对碱蓬根际土壤中根系分泌物含量、微生物数量、微生物多样性及根系吸收面积的影响。结果表明:与对照相比,轻度盐胁迫下碱蓬根系分泌物中的有机酸总量、氨基酸总量、微生物量碳和氮含量及根系总吸收面积变化较小,微生物数量和根体积、根系活跃吸收面积显著下降,而中度、重度盐胁迫使上述指标均明显下降,且降幅随着盐胁迫梯度的增加而增大;当接种PGPR后,碱蓬根系分泌物中的有机酸总量、氨基酸总量、微生物数量和微生物量碳、氮含量以及根系总吸收面积、活跃吸收面积均呈上升趋势,其中轻度盐胁迫接种PGPR处理的上述指标均最高,并显著高于其他处理,且有机酸总量、细菌数、根系总吸收面积和活跃吸收面积分别显著高于CK 14.57%、12.80%、8.33%和28.24%。此外,轻度盐胁迫接种PGPR处理的微生物多样性指数最高,依次为对照、轻度、中度和重度盐胁迫处理,这与微生物总量的变化规律一致;而中度或重度盐胁迫接种PGPR处理的微生物多样性指数与微生物总量的变化趋势不一致。综上,轻度盐胁迫(盐分含量100 g/kg)下接种蜡样芽孢杆菌能显著改善碱蓬根际土壤的微环境特征,其影响效果显著优于中度或重度盐胁迫下接种蜡样芽孢杆菌。

    Abstract:

    In order to explore the effect of inoculating plant growthpromoting rhizobacteria(PGPR)on the microenvironmental characteristics of the rhizosphere soil of Suaeda glauce Bge under different salt stress condition,a pot experiment including different salt stress gradient(light salt stress,moderate salt stress,severe salt stress)and corresponding inoculating PGPR was conducted to evaluate the effects of different treatments on root exudates,microbial populations,microbial diversity and root absorption area of Suaeda glauce Bge.The results indicated that the contents of organic acid and amino acid in the exudates,and microbial biomass carbon,microbial biomass nitrogen as well as total root absorption area had no significant differences with CK treatment,and the microorganism population,root volume and active absorption area under light salt stress conditions were significantly decreased,whereas the above indexes were all significantly decreased under moderate or severe salt stress conditions and the amplitude of reduction become larger along with the increase of degree of salt stress.After inoculating PGPR,the organic acid and amino acid contents in the exudates,and microbial biomass carbon and microbial biomass nitrogen contents as well as total absorption area and active absorption area of root system presented a trend of increasing,in which the above indexes achieved the maximum value by LS+P treatment and had significantly differences with the other treatments,showing 1457%,1280%,833% and 2824% increases in organic acid content,bacteria population,total absorption area and active absorption area of root system in comparison with CK treatment.Additionally,the microbial diversity index was the highest in LS+P treatment,followed by CK,light,moderate and severe salt stress,which had the same variation tendency as total microbial population.However,the microbial diversity indices and microbial population had different variation tendency between inoculating PGPR under moderate or severe salt stress and total microbial population.As a result,inoculating PGPR under light salt stress(salt content 100 g/kg)could significantly improve the microenvironmental characteristics of rhizosphere soil of Suaeda glauce Bge and the action effect was significantly higher than inoculating PGPR under moderate salt stress and severe salt stress.

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井大炜,马海林*,刘方春,杜振宇,贾海慧,马丙尧,王春丽,郭英华,丁洪星.盐胁迫环境下接种根际促生细菌对碱蓬根际土壤微环境特征的影响[J].中国土壤与肥料,2018,(4):34-39.

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  • 在线发布日期: 2018-08-17
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