In order to study the effect of combined application of organic and inorganic fertilizers on the bacterial communitystructure of tobacco growingsoil，through 3 years -locatedexperiment，the effects of chemical fertilizer(CF)，tobacco straw bio-organic fertilizer combined with chemical fertilizer(TBF)and high -carbon-based soil remediation fertilizercombined with chemicalfertilizer(HCF)on soil bacterial diversity and community structure were analyzed by the Illumina MiSeq high-throughput sequencingtechnology，and the important influence factors for bacterial community changes underdifferent fertilization conditions were explored combined with soil properties. Compared with CFtreatment，the content of alkaline nitrogen in soil treated by HCF was significantly reduced by 34.09%. The diversity index analysis showedthat organic and inorganic fertilizers could improve soil bacterialdiversity，and HCF treatment had the highest bacterialdiversity. Combined application of organic and inorganic fertilizers increased the bacterial abundance ofActinobacteria，Proteobacteria，Acidobacteria，Gemmatimonadetes，Bacteroidetes andPlanctomycetes，reduced the bacterial abundanceofChloroflexi，Saccharibacteria，Firmicutes，WD272 and Cyanobacteria. Organic and inorganic fertilizers combined application significantly increased the abundance of beneficial bacterial such as Patulibacter，Brevibacillus and Niastella.Spearman correlation analysis and CCA showed that soilpH，alkaline nitrogen and available potassium were important influence factors affecting soil bacterial community structure. The combined application of organic and inorganic fertilizershas a profound impact on bacterial diversity and community structure in tobacco growing soil.SoilpH，alkaline nitrogenand available potassium are important factors influencing the structure of bacterial community，which should be paid more attention in soil fertilization.