The vermicompost obtained from semi-fermented cow dung and mushroom residue was mixed with meteorite in different proportions to be used as the seedling substrate of tomato in order to study the effect of vermicompost substrate on the growth and development of tomatoseedlings，facilitate the development of new substrates for vegetable seedlings and providereliable，inexpensive，and widely used seedling substrates for vegetable factory seedlings. The results show that the bulk density of each treatment substrate is between 0.43～ 0.79 g/cm3，the total porosity is between 65.32%～72.95%，the size-to-void ratio is between 1∶1.53～ 1∶2.02，the pH is between 5.45～8.75，and the EC value is between 0.91～ 1.38mS/cm，other physical and chemical properties are in a suitable range except for the pH value. Compared with thecontrol，the vermicompost substrates have higher pHvalue，but has no effect on the final emergence of the tomato. The vermicompostsubstrates promoted the growth and development of tomatoseedlings，and its effect was related to the proportion of substratemixing. The effect of vermicompost:meteorite=2∶1(V∶V)was the best. Compared with thecontrol，the plantheight，stem diameter，dry weight of theunderground，dry weight of the whole plant and seedling index increased significantly by 23.12%，11.76%，57.14%，38.24% and60.34%，respectively( P<0.05).