Taking albic soil samples respectively from corn intercropping with Medicago sativa L.(hereinafter referred to ascornintercropping)and corn monocropping under the 3 -year field experiment as the researchobjects，the corn monocroppingserving as acontrol，the effect of corn intercropping on the humus composition of albic soil was explored under different levelsof Napplication.Four N application levels of140，175，200 and 225 kg/hm² were set up to explore the dynamic changes oftotal organicC(TOC)content，C contents of water -solublesubstance(WSS)，humicacid(HA)，humic -extracted acid(HE)，and humin(Hu)components(C WSS，CHA，CHE and CHu)，C HA/CFA ratio and E4/E6 ratio of HA alkaline solutionof albic soil from two cultivation modes at the cornjointing，tasseling，and fillingstages，respectively.The results showedthat:(1)Regardless of corn monocropping or intercropping with Medicago sativa L.，the two cultivation modes combined with N application of 200 kg/hm²fully inhibited the microbial mineralization and decomposition ofTOC(6.69 and 6.93g/kg，respectively)at the corn filling stage，which was significantly higher than that of othertreatments；(2)Corn monocropping or intercropping combined with 140 kg/hm² of N application significantly increased CHuat the filling stage，and the ratio of CHEin TOC was also significantly increased；(3)The corn monocropping combined with higher N application levels(200 and 225 kg/hm²)，and the intercropping combined with lower N application levels(140 and 175 kg/hm²)were all beneficialto the conversion of FA to HA and improving the humusquality，especially at the tasseling and fillingstages.Combined with N application at 200 kg/hm²，the formation of HA could be more promoted，and C HA was 1.85 and 1.74 g/kg at the tasselingand fillingstages，respectively，which was significantly higher than othertreatments.Under the influence of Napplication， the intercropping with Medicago sativa L.was easier to promote the degree of aromatization of HAmolecules.