This experiment was carried out to study the effects of crop rotation and continuous cropping to N2O emissioncharacteristics in yellow soil tobacco fields.N 2O emission flux was determined using a static chamber-gas chromatograph technique on a long-term test station in Longgang Town Tobacco Science Institute of GuizhouProvince.The experiment wasset up with threetreatments，including no fertilizer + crop rotation forcomparison(CK)；inorganic fertilizer + crop rotation(CR)；inorganic fertilizer + continuouscropping(CC).The results showed that tobacco growth season of N 2O emissionpeaks was appeared at 3～ 5 d after basalfertilizer，and 1～ 7 d after topfertilizer，respectively.After basal fertilizer the total cumulative N2O emissions accounted for high percentage(16.26%～18.30%)of the whole growingseason.The order of the average N2O emission flux among treatments was CC>CR>CK in the tobacco growing season，which was 0.044 and 0.022 and 0.013 mg·m-2·h-1，respectively.Under the condition of same fertilization，compared with rotation，the N 2Oemission of continuous cropping increased 2times，and tobacco biomass decreased by30.01%.Correlation analysis showed that N2O emission flux of inorganic fertilizer + crop rotation was mainly associated with soil nitrate nitrogen content in Yellow-soil tobaccofields( P<0.05).N2O emission was relatively high at tobacco continuous cropping in yellow soil tobaccofields. Rotation was the main cause significantly decreased of N2O emission in tobaccogrowth，reasonable crop rotation was the best way to reduce N2O emissions in yellow soil tobaccofields.