施氮和间作花生对木薯根际土壤细菌群落结构的影响
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(江西省农业科学院土壤肥料与资源环境研究所 /农业农村部长江中下游作物生理生态与.耕作重点实验室,江西 南昌 330200)

作者简介:

林洪鑫(1983-),男,江西兴国人,助理研究员,博士,从事作物高产栽培研究。E-mail:lhxtfs@163.com。

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基金项目:现代农业产业技术体系建设专项“国家木薯产业技术体系南昌综合试验站”(CARS-11-JXLHX)。


Effects of nitrogen application and intercropping with peanut on bacterial community structure in cassava rhizosphere soil
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(Soil and Fertilizer& Resources and Environment Institute,Jiangxi Academy of Agricultural Sciences/Key Laboratory of Crop Ecophysiologyand Farming System for the Middle and Lower Reaches of the Yangtze River,Ministry of Agriculture and Rural Affairs,Nanchang Jiangxi330200)

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    摘要:

    在等量施肥条件下,木薯间作一季花生,是否具有间作优势,间作模式中木薯根际土壤细菌群落结构如何变化,相关研究报道较少。以木薯品种“华南 205”、花生品种“粤油 200”为试验材料,设计施氮、不施氮 2个处理和等量施肥条件下的 3种种植模式(木薯单作、花生单作、木薯间作花生),采用高通量测序技术,研究施氮和间作花生对木薯根际土壤细菌群落结构的影响。结果表明,在等量施肥条件下,木薯 -花生间作的土地当量比大于 1,表现出间作优势。施氮和间作花生对木薯根际土壤细菌的多样性无显著影响。4个优势细菌门类依次为绿弯菌门、变形菌门、放线菌门和酸杆菌门。不同处理间的菌门 Saccharibacteria的差异极显著,施氮显著降低了疣微菌门的相对丰度。木薯根际土壤细菌的属数量共 464个。相对丰度排名前 30的菌属中,不同处理间菌属 Acidobacteriaceae__Subgroup_1_和 Sphingomonas的差异达显著或极显著水平,施氮显著降低了菌属 Subgroup_7的相对丰度,显著提高了菌属 Acidothermus的相对丰度。主成分分析表明,施氮对细菌群落组成的影响大于种植模式;冗余分析得出,有机质、pH值显著影响细菌属水平群落组成。

    Abstract:

    Under the condition of equal amount fertilization,whether cassava intercropping with one season peanut hasthe intercropping advantage ornot,and how the bacterial community structure of cassava rhizosphere soil changes in theintercropping pattern,little related research on these questions are reported.Using cassava variety“South China 205”and peanut variety“Yueyou 200”as experimental materials,designing two levels as with nitrogen application and withoutnitrogen application,and three planting patterns(cassava mono -cropping,peanut mono -cropping,cassava intercroppingwith peanut)under the conditions of equal amountfertilization,an experiment was designed to analyze the effect of cassava -peanut intercropping and nitrogen application on the bacterial community structures in cassava rhizosphere soil in Jiangxi province based on high-throughput sequencingtechniques.The results showed that under the condition of equalfertilization, the land equivalent ratio of cassava-peanut intercropping was more than1,showed the intercroppingadvantage.Nitrogenapplication and intercropping with peanut had no significant effect on the diversity of bacteria in cassava rhizosphere soil.The four dominant bacteria were Chloroflexi,Proteobacteria,Actinobacteria and Acidobacteria in turn.The differenceof Saccharibacteria among different combinations of nitrogen application and planting patterns was extremelysignificant.Nitrogen application significantly reduced the relative abundance ofVerrucomicrobia.There are 464 bacteria genera in therhizosphere cassavasoil.Among the top 30 genera in relative abundance,the difference of Acidobacteriaceae__Subgroup_1_ and Sphingomonas among different combinations of nitrogen application and planting patterns was significant or extremelysignificant.Nitrogen application significantly reduced the relative abundance of Subgroup_7 and increased the relative abundance of Acidothermus.Planting patterns had no significant effect on the relative abundance of soil bacteria in cassavarhizosphere.The bacterial community structure of cassava rhizosphere soil under the condition of equal amount fertilizationwas studied to provide a theoretical basis for clarifying the rhizosphere microecology of cassava in intercropping pattern. Principal component analysis showed that the effect of nitrogen application on bacterial community composition was greaterthan that of plantingpattern.Redundancy analysis showed that organic matter and pH significantly affected the compositionof bacterial horizontalcommunity.

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林洪鑫,袁展汽,肖运萍,汪瑞清,吕丰娟,张志华.施氮和间作花生对木薯根际土壤细菌群落结构的影响[J].中国土壤与肥料,2020,(4):56-65.

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  • 收稿日期:2019-06-25
  • 最后修改日期:
  • 录用日期:2019-08-04
  • 在线发布日期: 2020-09-14