Under the condition of equal amount fertilization，whether cassava intercropping with one season peanut hasthe intercropping advantage ornot，and how the bacterial community structure of cassava rhizosphere soil changes in theintercropping pattern，little related research on these questions are reported.Using cassava variety“South China 205”and peanut variety“Yueyou 200”as experimental materials，designing two levels as with nitrogen application and withoutnitrogen application，and three planting patterns(cassava mono -cropping，peanut mono -cropping，cassava intercroppingwith peanut)under the conditions of equal amountfertilization，an experiment was designed to analyze the effect of cassava -peanut intercropping and nitrogen application on the bacterial community structures in cassava rhizosphere soil in Jiangxi province based on high-throughput sequencingtechniques.The results showed that under the condition of equalfertilization， the land equivalent ratio of cassava-peanut intercropping was more than1，showed the intercroppingadvantage.Nitrogenapplication and intercropping with peanut had no significant effect on the diversity of bacteria in cassava rhizosphere soil.The four dominant bacteria were Chloroflexi，Proteobacteria，Actinobacteria and Acidobacteria in turn.The differenceof Saccharibacteria among different combinations of nitrogen application and planting patterns was extremelysignificant.Nitrogen application significantly reduced the relative abundance ofVerrucomicrobia.There are 464 bacteria genera in therhizosphere cassavasoil.Among the top 30 genera in relative abundance，the difference of Acidobacteriaceae__Subgroup_1_ and Sphingomonas among different combinations of nitrogen application and planting patterns was significant or extremelysignificant.Nitrogen application significantly reduced the relative abundance of Subgroup_7 and increased the relative abundance of Acidothermus.Planting patterns had no significant effect on the relative abundance of soil bacteria in cassavarhizosphere.The bacterial community structure of cassava rhizosphere soil under the condition of equal amount fertilizationwas studied to provide a theoretical basis for clarifying the rhizosphere microecology of cassava in intercropping pattern. Principal component analysis showed that the effect of nitrogen application on bacterial community composition was greaterthan that of plantingpattern.Redundancy analysis showed that organic matter and pH significantly affected the compositionof bacterial horizontalcommunity.