TakingSoybean(VignaNo.1)as the researchobject，six strains with colonyuplift，neat edges，mucus and high nitrogenase activity were identified from the nodule samples collected in different areas of Guizhou and madeinto a composite inoculant. To analyze the effects of different combinations on soybean plant height，root length，nodulenumber，above-ground and underground biomass production，the nutrient qualities including crude protein(CP)，neutral fiber(NDF)，acid fiber(ADF)，total phosphorus(P)and calcium(Ca)were determined to select the bestcombination strains with symbiotic matching with soybean plants. The results showed that 4 of them wererhizobium(R286 -1， R287-3，R310-1，R325-3)，2 strains were endophytic bacteria(R286 -5，R287-5). Compared with single inoculationrhizobium treatment，most combination inoculants significantly promoted the stem diameter of soybean，the dry matterweights of ground part ofB2，D1 and D2 were the mostobvious，and the increases of root biomass ofA1，B1 and D1 werethe most effective.Preferably，the treatments ofA2，B2，C1 and D1 had more nodule numbers. Adding endophytic bacteriasignificantly increased the CP content ofplants，the composite bacteria increased by 4.6%～ 13.1% thanCK，the calciumcontent decreased by 11.3% forD1，and the other combinations increased by 4.1%～20.8%，which significantly reducedthe ADF content(except forA1，B1，B2，D1). Based on the comprehensive analysis，the application of the four mostproductive groups ofA2，B2，C1 and D2 laid the experimental basis for the development of soybean fertilizer.