牧草促生菌分离鉴定及对大豆促生性能的研究
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(贵州省农业科学院草业研究所,贵州 贵阳 550006)

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舒健虹(1972-),女,湖南长沙人,高级农艺师,本科,主要从事牧草育种与生物技术研究。E-mail:gzsjhong@126.com。

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基金项目:贵州省科技支撑计划(黔科合支撑[2016]2504号);国家自然科学基金项目(31560035);贵州省科技支撑计划(黔科合支撑[2019]2359号)。


Identification of forage-promoting strains and their effects on soybean growth and quality
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(Institute of Grass Industry of GuizhouAcademy of Agricultral Science,Guiyang Guizhou550006)

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    摘要:

    以大豆(黔豆 1号)为研究对象,从贵州不同地区采集的根瘤样品中,挑选菌落隆起、边缘整齐、有粘液,且固氮酶活性较高的 6个菌株进行鉴定并制成复合接种剂,分析不同组合处理对大豆的株高、根长、根瘤数、地上与地下部分生物产量等的影响,测定了营养品质粗蛋白(CP)、中性纤维(NDF)、酸性纤维(ADF)、全磷(P)、钙(Ca)的含量,旨在从中筛选出与大豆植株共生匹配最佳的组合菌株。结果表明:经鉴定其中 4株是根瘤菌(R286-1、R287-3、R310-1、R325-3),2株为内生细菌(R286-5、R287-5)。与单一接种根瘤菌处理相比,大多组合接种剂可显著促进大豆的茎粗,地上部分干物质重量 B2,D1和 D2的效果最明显,A1、B1和 D1增加根系生物量的效果最佳,根瘤数 A2、B2、C1和 D1结瘤最多。加施内生细菌能显著提高植物的 CP含量,复合菌剂比 CK提高 4.6%~13.1%,Ca含量仅 D1比 CK下降11.3%,其他组合提高 4.1%~20.8%,明显降低了ADF含量(除A1、B1、B2、D1)。综合分析,应用生产潜力最大的 4个菌组为A2、B2、C1、D2,为大豆菌肥的研制奠定试验基础。

    Abstract:

    TakingSoybean(VignaNo.1)as the researchobject,six strains with colonyuplift,neat edges,mucus and high nitrogenase activity were identified from the nodule samples collected in different areas of Guizhou and madeinto a composite inoculant. To analyze the effects of different combinations on soybean plant height,root length,nodulenumber,above-ground and underground biomass production,the nutrient qualities including crude protein(CP),neutral fiber(NDF),acid fiber(ADF),total phosphorus(P)and calcium(Ca)were determined to select the bestcombination strains with symbiotic matching with soybean plants. The results showed that 4 of them wererhizobium(R286 -1, R287-3,R310-1,R325-3),2 strains were endophytic bacteria(R286 -5,R287-5). Compared with single inoculationrhizobium treatment,most combination inoculants significantly promoted the stem diameter of soybean,the dry matterweights of ground part ofB2,D1 and D2 were the mostobvious,and the increases of root biomass ofA1,B1 and D1 werethe most effective.Preferably,the treatments ofA2,B2,C1 and D1 had more nodule numbers. Adding endophytic bacteriasignificantly increased the CP content ofplants,the composite bacteria increased by 4.6%~ 13.1% thanCK,the calciumcontent decreased by 11.3% forD1,and the other combinations increased by 4.1%~20.8%,which significantly reducedthe ADF content(except forA1,B1,B2,D1). Based on the comprehensive analysis,the application of the four mostproductive groups ofA2,B2,C1 and D2 laid the experimental basis for the development of soybean fertilizer.

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舒健虹,王子苑,刘晓霞,曾庆飞,王小利.牧草促生菌分离鉴定及对大豆促生性能的研究[J].中国土壤与肥料,2020,(4):215-222.

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  • 收稿日期:2019-08-28
  • 最后修改日期:
  • 录用日期:2019-11-09
  • 在线发布日期: 2020-09-14