Pot experiment wasadopted，and the responses of summer maize root dehydrogenaseactivity，the nutrient uptakeofN，P andK，dry matter accumulation and yield to different fertilization methods were analyzed to provide theoreticalbasis for rational fertilization of summermaize.Results showed that compared with the traditionalfertilization(T6)，T4(the recommended fertilization amount is divided into 2layers)was thebest.The dry matter accumulation of T4 increasedby30.4%，and the nutrient uptake ofN，P and K increased by30.6%，28.5% and26.4%，respectively，and the yieldincreased by 34.3%.T5(the recommended fertilization amount is divided into 3 layers)was the second.The dry matteraccumulation of T5 increased by13.1%，the dehydrogenase activity of 25～ 35 cm increased by40.3%，and the nutrientuptake of N and K increased by 5.7% and7.7%，respectively，and the yield increased by16.0%.The recommendedfertilization combined with organic fertilizer can meet the nutrient requirements of maize in the latestage，promote the rootsystemdevelopment，effectively promote the dehydrogenase activity of roots in various soillayers，increase the accumulationof dry matter andN，P and Knutrients，and increase the yieldsignificantly.Overall，T4(half of the recommended amount of N fertilizer applied to 5～15cm，the other half of the N fertilizer + the total P and K fertilizer + bio -organic fertilizerapplied to 15～ 25cm)processing is moreappropriate.