PGPR菌剂对辣椒的促生效应及根际土壤细菌的响应研究
作者:
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(1.贵州大学生命科学学院,贵州 贵阳 550025;2.贵州省农业科学院辣椒研究所,贵州 贵阳 550006)

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黄文茂(1993-),男,贵州遵义人,在读硕士研究生,研究方向为农业及环境微生物。E-mail:tfzhwm@126.com。

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基金项目:国家自然科学基金项目(31760030);贵州省科技计划项目(黔科合平台人才 [2017]5788);贵州省科技计划支撑项目(黔科合支撑[2017]2575)。


Growth-promoting effects of PGPR microbial agent on chilli and response of rhizosphere soil bacteria
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(1.College of LifeSciences,GuizhouUniversity,Guiyang Guizhou550025;2.Pepper Research Institute,Guizhou Academy of Agricultural Sciences,Guiyang Guizhou550006)

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    摘要:

    研究田间条件下 PGPR菌剂灌根对辣椒根际土壤细菌数量及群落结构的影响,为深入探究 PGPR菌剂的田间促生机制提供理论依据。以 4株 PGPR菌株制成 PGPR菌剂,对辣椒进行灌根,并采用 Illumina-MiSeq平台对辣椒根际土壤细菌 V3-V4区进行 Paired-end测序,结合细菌及功能菌群数量、辣椒产量指标,比较分析菌剂灌根配施全量化肥(PGPR-CF1)、菌剂灌根配施 80%化肥(PGPR-CF2)和全量化肥(CK)处理后对辣椒根际土壤细菌数量及群落结构特征的影响。结果表明:PGPR菌剂灌根后辣椒根际土壤细菌及包含固氮、解磷及解钾等功能菌群的数量显著提高,且物种丰度和多样性均高于对照,细菌优势度则表现为 PGPR-CF1和 CK组高于 PGPR-CF2。所有土样共得到有效序列 573 896条,在相似水平为 97%下聚类分析得到 OTUs数分别为 2 153个(CK)、2 337个(PGPR-CF1)、2 358个(PGPR-CF2);各处理辣椒根际土壤中的优势门为酸杆菌门、放线菌门、拟杆菌门、绿弯菌门、浮霉菌门和变形菌门,相对丰度总占比分别为 83.7%(PGPR-CF1)、79.55%(PGPR-CF2)和 79.77%(CK)。PGPR菌剂灌根后增加了伯克霍尔德氏菌目、芽孢杆菌目、鞘脂单胞菌目等的相对丰度,降低了芽单胞菌目、酸杆菌目的相对丰度。Pearson指数表明,辣椒产量和单果重与根际土壤细菌多样性和丰度正相关。研究显示,PGPR菌剂处理,可以提高根际土壤细菌和功能菌群的数量,同时影响了物种多样性和群落结构,并促进了辣椒增产。

    Abstract:

    The effects of PGPR inoculants on the number and community structure of bacteria in chilli rhizosphere soil under field conditions are investigated to provide a theoretical basis for further exploring growth-promoting mechanism of theseinoculants. Three treatments aredesigned,including PGPR inoculants plus 100% chemical fertilizer(PGPR -CF1),PGPRinoculants plus 80% chemical fertilizer(PGPR -CF2)and 100% chemical fertilizer(CK). The PGPRinoculants,consistedof four bacterial strains,are irrigated to chilli ’s roots. And then V3-V4 region of 16S rRNA gene in the rhizosphere is sequenced by Paired-end using Illumina-MiSeqplatform,combining with measurements of functional bacteria numbers and yield indexes of chilli. The effects on bacteria numbers and community structure in chilli rhizosphere soil are compared andanalyzed based on above results. The result shows that the number of bacteria and functionalbacteria,including nitrogen -fixingbacteria,phosphorus -dissolving bacteria and potassium-insolubingbacteria,increase significantly after root irrigationwith PGPR,the bacterial abundance and diversity in group treated with PGPR inoculants are higher than controlgroup,but the bacterial dominance of PGPR-CF1 and CK are higher than PGPR-CF2. Under the similarity level of97%,the OTUsnumber ofCK,PGPR -CF1 and PGPR-CF2 are 2153,2 337 and 2358,respectively,by cluster analysis of 573 896valid sequences;The dominant phylum in the chilli rhizosphere soil of all treatments areAcidobacteria,Actinobacteria,Bacteroidetes,Chloroflexi,Planctomycetes,Proteobacteria. The relative abundance ratios were 83.7%(PGPR -CF1),79.55%(PGPR -CF2)and79.77%(CK),respectively.However,the relative abundance of orders such asBacillales,Burkholderiales,Sphingobacteriales,etc.,are increased after the irrigation with PGPRinoculants,the otherorders,suchasGemmatimonadales,Actinomycetes,etc.,are the opposite. Chilli yield and weight of single fruit are positively correlated with the diversity and abundance of bacteria in the chilli rhizosphere soil based on Pearson indexes. PGPR inoculants improvenumbers of bacteria and functional bacteria in rhizosphere soil,impact on the rhizobacterial diversity and communitystructure,and increase the yields of chilli.

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黄文茂,詹永发,王欢,韩丽珍. PGPR菌剂对辣椒的促生效应及根际土壤细菌的响应研究[J].中国土壤与肥料,2020,(5):152-160.

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  • 收稿日期:2019-08-03
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  • 录用日期:2019-08-31
  • 在线发布日期: 2020-11-06