To discuss the effects of spraying iron fertilizer on vegetable growth and microbial diversity in karstareas，a pot experiment was conducted to study the effects of different iron fertilizers onbiomass，leaf antioxidantenzymes，soil nutrients and microbes ofcabbage.The experiment consisted of six different treatments，includingwater(CK)，ferrous sulfate with 0.05%Fe(Y0)and different organic iron fertilizer with0.05%，0.03%，0.025% and 0.02%Fe(marked asY1，Y2，Y3andY4).The results showed that 0.05% FeSO 4reduced the aboveground biomass ofcabbage，which was 14.16% less thanthecontrol.Organic iron fertilizer promoted the growth ofcabbage，Y3 and Y4 treatments significantly increased biomassby 54.26% and57.86%，respectively.Compared withCK，organic iron fertilizer increased or significantly increasedleaf antioxidant enzyme activity.While，SOD had the most obvious change.Y3 and Y4 reduced the malondialdehyde(MDA)content，while the other treatments increased MDA.As the concentration of organic iron fertilizerdecreased，thecontents of soil alkaline nitrogen(AN)，available phosphorus(AP)and available potassium(AK)reduced，while soilorganicmatter(SOM)increased.Soil nutrients had significantly difference in Y4 compared withCK.Y4 showed thehighest amount ofbacteria(4.27 nmol·g -1)，fungi(0.85 nmol·g -1)andactinomycetes(0.39 nmol·g -1).However，Y1 showed the lowest amount ofbacteria(1.18 nmol·g -1)，fungi(0.20 nmol·g -1)andactinomycetes(0.12 nmol·g -1).Principal componentanalysis(PCA)showed a significant difference in soil microbial community between ferrous sulfatetreatment and organic iron fertilizertreatment. Y0 treatment increased the multi-degree value of Gram-negative bacteria(16∶1ω9c，18∶1ω7c)andactinomycetes(10Me17∶0，10Me18∶0)，Y3 and Y4 treatments increased the multi -degree value of Gram-positivebacteria(a17∶0，i18∶0).Pearson correlation analysis of yield and multiple factors indicated that yieldwas significantly negatively correlated with MDA( P<0.05)andAK( P<0.01).Additionally，there was a significant positivecorrelation between yield withSOM( P<0.05).However，there was no significant correlation between yield withmicroorganisms.In a comprehensivecomparison，0.02% organic iron fertilizer had the best effect on the cabbage yield and soil microbial diversity inkarstarea.The research can provide reference for the development of ecological agriculture in karstarea.