A Penicillium brocae strain P6 which could form transparent halos on Ca3(PO 4)2 -containing Pikovskaya solidmedia was isolated from soil，while no transparent halos could be observed by naked eyes on FePO 4 or AlPO4 -containing solid media with P6.The insoluble phosphate solubilizing capacities of P6 were also characterized in liquid media. P6 solubilized Ca3(PO 4)2 efficiently and it also showed solubilizing capacity to FePO4 and AlPO4 but not comparable to Ca3(PO4)2.Nitrogen resource forms could affect the phosphate solubilization capacity ofP6.Data analysis unveiled thatpH of the P6 cultivated media deceased as the elongation of cultivationtime.Oxalicacid，malicacid，lactic acid and citric acid were detected by High-Pressure LiquidChromatography(HPLC)in P6 fermented media but no correlation were found between organic acids concentration and Ca3(PO4)2solubilizing capacities.Additionally，pot experiment showed that P6 had growth-promoting effects on Brassica chinensis.The B.chinensisfreshweight，dry weight and rhizosphere soil availablephosphorus ofT2(Calcium -magnesia phosphate fertilizer+ P6 spores suspension)were higher than that of CK1(water)，CK2(Calcium-magnesia phosphatefertilizer)andT1(P6 sporessuspension)，respectively.Although the indexes aboveof T1 were not as good asCK2，the B.chinensis fresh weight and rhizosphere soil available phosphorus of T1 were higherthan that ofCK1.It indicated that P6 can increase soil available phosphorus and promote the growth of B.chinensis.