The influences of selenium fertilizer applied in soil on the physiological characteristics during flowering and pod setting stages and the agronomic and yield characteristics for peanut of different varieties with plastic film were studied.The results indicated that selenium fertilizer applied in soil had little effect on both the physiological characteristics during the pod setting stage and the agronomic ones of the 3 peanut varieties，while both the physiological characteristics during flowering stage and the yield ones such as the 100-pod weight，the plump pod rate，the number and productivity per plant of the 3 varieties with selenium fertilizer applied in soil were significantly higher than those of CK，but there was difference of the values on these characteristics among the different peanut varieties.The content of chlorophyll a and the SOD，CAT activities in leaves of Luhua 4 with selenium fertilizer applied in soil were all significantly higher than those of CK during flowering stage，and its number and productivity per plant were respectively 52.4% and 90.8% higher than those of CK(respectively 12.8 and 22.7 g).The content of chlorophyll a，chlorophyll b，chlorophyll a and b，and the SOD，CAT activities in leaves of Huayu 16 with selenium fertilizer applied in soil were all significantly higher than those of CK during flowering stage，too，and its values on the yield characteristics were slightly higher than those of CK.And both the content of chlorophyll a and chlorophyll a and b in leaves of the black peanut variety Heiyuzhen with selenium fertilizer applied in soil were significantly higher than those of CK during flowering stage，and its 100-pod weight，plump pod rate，and productivity per plant were respectively 166.9 g，95.2% and 20.5 g，which were respectively 37.5%，4.73% and 64.0% higher than those of CK.The results of the correlation coefficient analyzed among the physiological characteristics during flowering stage and the yield ones furtherly showed that the content of chlorophyll a and the ratio of.chlorophyll a.to b in leaves showed much positively correlated with the number and productivity per plant，and what’s more，the coefficient between the ratio of.chlorophyll a.to b and the number per plant was at a significant level(0.9257).These indicated that the average values of both the photosynthetic characteristics and the antioxidative enzymes’ activities during flowering stage of the 3 peanut varieties with plastic film were improved by applying selenium fertilizer in soil，and so the average values of their number and productivity per plant and yield were increased，which provided some theoretical basis for high yield and high efficiency cultivation techniques of peanut enriched with selenium.It is suggested that suitable matching application technologies of selenium fertilizer should be adopted according to different peanut varieties in order to have the best effect on decreasing cost and increasing benefit in practice.