Three-year-old field-cultivated ‘Summer black’ grape were used as test crop，and the spraying concentrations were 0 mg·L-1(CK)，20 mg·L-1(T1)，40 mg·L-1(T2)，60 mg·L-1(T3)and 80 mg·L-1(T4)sodium selenite solutions to investigate their effects on the physiological properties and fruit quality of grape.The results showed that:1)Spraying sodium selenite had effects on the physiological characteristics of grapes.The soluble protein content，proline content，SOD，POD，and CAT activities of leaves increased first and then decreased with the increase of sodium selenite concentration，and reached the maximum when the sodium selenite concentration was at 40 mg·L-1；Compared with CK，it was significantly increased by 11.27%，11.89%，10.67%，8.33% and 15.60%，respectively.The relative conductivity and MDA content of each treatment leaf were significantly reduced by 7.73%，14.39%，respectively，at the concentration of 40 mg·L-1 sodium selenite.2)The single grain weight，single ear weight，fruit shape index，soluble protein，and total phenol content of 40 mg·L-1 sodium selenite treatment increased by 8.81%，6.82%，7.06%，6.93% and 24.43%，respectively.The ratios of solid acid to sodium selenite at 40 and 80 mg·L-1 were significantly increased by 8.05% and 7.95% compared with CK.The contents of Vc were significantly increased by 7.52%，7.60% when the sodium selenite was 40 and 60 mg·L-1.When the concentration of sodium selenite was 60 mg·L-1，the contents of flavonoids and anthocyanins in fruits were significantly increased by 20.14% and 18.80%，respectively.The fruit selenite content was positively correlated with the concentration of sodium selenite treatment，which were 1.75，3.64，6.69 and 8.92 times that of CK，respectively.The results of principal component analysis showed that the order of improving the physiological characteristics and improving fruit quality of the five selenium application levels was T2>T3>T1>T4>CK，and the comprehensive analysis showed that 40 mg·L-1 sodium selenite application had the best effect.