多功能地衣真菌的筛选及其复合菌剂的研制
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(山东师范大学生命科学学院,山东 济南 250014)

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吴晓华(1999-),女,山东泰安人,本科,研究方向为微生物资源与利用。E-mail:3208388207@qq.com。张晓坤(1998-),女,山东青岛人,本科,研究方向为微生物资源与利用。E-mail:2667563408@qq.com。张晓坤为共同第一作者。

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基金项目:国家自然科学基金(31600100);山东省自然科学基金(ZR2014CM025);校级大学生创新训练项目(201910445511)。


Screening of multifunctional lichen fungi and development of compound inoculant
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(College of Life Sciences,Shandong Normal University,Jinan Shandong 250014)

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    摘要:

    为了从地衣真菌中筛选高效多功能菌种,研制出高效的微生物菌肥,对地衣真菌采用水解圈法初筛,再分别采用DNS法、钼锑抗比色法、平板对峙法复筛出具有高效降解纤维素、溶解无机磷、抑制病原菌活性的菌株;以不同的菌株组合研制生物菌剂,并进行盆栽试验筛选出促生活性最好的组合。从120株地衣真菌中筛选出高效降解的纤维素菌株为1905-2、1909-7、1913-6、1915-2,其中菌株1905-2和1915-2兼具有抑制病原菌的活性;高效溶磷菌株为1905-4,同时具有抑制病原菌的活性。结合形态学及分子序列鉴定菌株1905-2为高山弯颈霉(Tolypocladium tropicale),1905-4为塔宾曲霉(Aspergillus tubingensis),1909-7为黄曲霉(Aspergillus aflatoxiformans),1913-6为产黄青霉(Penicillium chrysogenum),1915-2为淡紫拟青霉菌(Purpureocillium lilacinum)。组合5(1905-4、1915-2)在增加茎长和鲜重上占有优势,且溶磷效果及降解纤维素效果最好,因此综合选取以组合5研制的生物菌剂作为最终的微生物菌剂。从地衣真菌筛选出高效功能菌株,复合后研制出具有高效降解纤维素、溶解无机磷、抑制病原菌的复合菌剂,旨在为微生物资源的开发与利用、探究研制高产的生态菌肥提供理论基础。

    Abstract:

    In this study,high efficient and multifunctional strains were screened from lichen fungi,and high efficient microbial fertilizer was developed.Hydrolytic circle method was used at the initial screening,then DNS method,molybdenum-antimony colorimetric method and plate antithetic method were used to qualitative determine the strains with high efficiency in degrading cellulose,dissolving inorganic phosphorus and inhibiting pathogen activity.Different combinations of strain were used to develop microbial inoculant,and pot experiments were carried out to select the best combination for promoting growth.Efficiently degrading cellulose strains from 120 lichen fungi were 1905-2,1909-7,1913-6 and 1915-2.Among them,strains 1905-2 and 1915-2 had the activity of inhibiting pathogenic fungus.Efficiently dissolving phosphorous strain was 1905-4,which also had the activity of inhibiting pathogen.Combined with morphological and molecular identification,strains 1905-2 were identified as Tolypocladium tropicale,1905-4 as Aspergillus tubingensis,1909-7 as Aspergillus aflatoxiformans,1913-6 as Penicillium chrysogenum,and 1915-2 as Purpureocillium by combining morphology and molecular sequence Lilacinum.The combination 5 (1905-4,1915-2)had the advantages in increasing stem length and wet weight,and it had the best effect in degrading cellulose and dissolving phosphorus.Therefore,the combination 5 was selected as the final microbial inoculant.Selecting high-efficiency functional strains from lichen fungi and developing compound microbial inoculant with high efficiency in degrading cellulose,dissolving inorganic phosphorus and inhibiting pathogen can provide the theoretical basis for the development and utilization of microbial resources and the high-yielding ecological microbial inoculant.

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吴晓华,张晓坤,曲玉森,张耀昆,司红丽.多功能地衣真菌的筛选及其复合菌剂的研制[J].中国土壤与肥料,2021,(1):289-295.

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  • 收稿日期:2019-11-20
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  • 录用日期:2020-02-07
  • 在线发布日期: 2021-03-18