Arbuscular mycorrhizal (AM)fungi are ubiquitous soil microorganism in terrestrial ecosystem and are one of the important functional microorganisms in the soil-plant system.It can form a arbuscular mycorrhiza symbiotic system with plant roots by infecting the latter，which will significantly improve root morphology and nutrition，thereby improving the growth and development，yield，quality and stress resistance of host plant.At present，more than 13 genera and 54 species of AM fungi have been isolated from the roots rhizosphere soil of tobacco(Nicotiana tabacum L .)plant，which reveals that there are potential AM fungi resources for tobacco cultivation.This article focuses on the symbiosis effect of tobacco and AM fungi，summarizes the main factors that affect the AM fungi′s infection and colonization of tobacco roots，the role of AM fungi in tobacco growth，resistance physiology and quality were also elaborated.We also summarized the synergistic effect between PGPR and AM fungi，and outlined the shortcoming and prospects of it.All of this will provide reference for the application of mycorrhizal technology in tobacco cultivation.