In order to investigate the effects of biochar input on water and nutrient retention in gravel soil and the yield and quality of wine grape under the condition of organic and inorganic application，a 2-year site-specific experiment was conducted at the eastern foot of Helan mountain，and the physical and chemical properties of soil and biological responses of crops under different biochar input conditions were analyzed.The results showed that under organic and inorganic application conditions，with the increase of biochar application amount，the content of water-stable aggregates of >0.25 mm particle size increased significantly，but the content of water-stable aggregates of <0.25 mm particle size decreased significantly，and the soil structure improved significantly.The application of biochar over 6000 kg/hm² significantly increased the water storage of 0～80 cm soil by more than 30%，and significantly increased the content of soil organic matter，available nitrogen and available potassium.It significantly promoted leaf photosynthesis of wine grape，optimized ear length，thus increased grape yield and improved grape quality.The optimal biochar input in the theoretical yield simulation was 7260 kg/hm².Therefore，the application of biochar 7260 kg/hm² in the gravel soil wine vineyard at the eastern foot of Helan mountain，under the conditions of organic and inorganic application，can quickly construct the soil，protect soil water and nutrients，and is conducive to promoting the healthy and sustainable development of wine grape industry.