Effects of different kinds of microbial fertilizer and amounts on kiwifruit quality were studied using 3-year-old “guichang” kiwifruit as test material.Three microbial fertilizers(microbial fertilizer Ⅰ containing Bacillus amyloliquefaciens and Bacillus licheniformis，microbial fertilizer Ⅱ containing Bacillus Subtilis，microbial fertilizerⅢ containing Bacillus Subtilis and Bacillus licheniformis)treated in three ways(3，6 and 9 kg/plant)were designed with a control(no fertilizer).Effects of these microbial fertilizers and amounts were analyzed and the optimum treatment was proposed.The results showed that these different microbial fertilizers all significantly improved the nutrient quality such as soluble solid，Vc，reducing sugar，soluble sugar，soluble protein content and sugar-acid ratio.The effects of three kinds of microbial fertilizer on the fruits of kiwifruit was fertilizerⅠ＞fertilizer Ⅲ＞fertilizer Ⅱ.The main manifestation was the content of sugar and acids in their fruits，fertilizer Ⅰ had the most influence on the soluble sugar，titratable acid content，and sugar-acid ratio，which was highly improved compared with the CK(63.41%，-27.11%，139.38%)，fertilizer Ⅱ and fertilizer Ⅲ(21.00%，-15.53%，51.23%).In terms of fertilizer amount，the fertilizer amount of any kind of microbial fertilizer greater than 6 kg/plant can significantly promote the fruit quality，all fruit index increase with the increase of fertilizer amount，but there was no significant difference between 9 and 6 kg/plant.Therefore， applying 6 kg/plant of fertilizer Ⅰ(treatment combination M2)provided the best effect，the second was applying 6 kg/plant of fertilizer Ⅲ(treatment combination K2).