Two different methods for the determination of water-soluble fluorine in fertilizer and soil conditioner by ion chromatography and ion selective electrode were established.The pre-treatment conditions of the samples were optimized，and the results showed that 30 min ultrasound at room temperature was the best extraction condition.Both ion chromatography and ion selective electrode method showed good linearity，high precision and accuracy，and the standard recovery was between 91.0% and 108.0%.In addition，there was no significant difference in the determination results of the same sample，indicating that both methods could meet the analysis requirements of water-soluble fluorine content in fertilizer and soil conditioner.Compared with ion selective electrode method，ion chromatography has the characteristics of faster analysis speed and less interference factors.