Polycyclic aromatic hydrocarbons are important organic pollutants in livestock and poultry manure，my country has not yet established a standard method for the determination of polycyclic aromatic hydrocarbons in livestork and poultry manure，which restricts the safe use of livestock and poulty manure resources.Therefore，a new method for simultaneous determination of 16 polycyclic aromatic hydrocarbons in livestock and poultry manure was established by gas chromatography-mass spectrometry.The results showed that the 16 pdycyclic aromatic hydrocarbons showed good linear relationship in the range of 0.02～2.00 mg/L，the detection limit was 0.005～0.046 mg/kg，the relative standard deviation was 2.0%～9.8%，and the standard recovery was 67.5%～111.6%.The method has the advantages of high accuracy，high precision，low detection limit，and simple operation.It is suitable for quick screening，confirmation and quantitative analysis of polycyclic aromatic hydrocarbons in resource utilization livestock and poultry manure.