DMPP(3，4-dimethyl pyrazole phosphate)and NBPT(n-butylthio-phosphotriamine)combined with two kinds of urea(ordinary urea，PASP-urea)were added to different soils，in order to explore the effects on N2O emission from purple soil of Sichuan，yellow brown paddy soil of Hubei and blue mud paddy soil of Zhejiang.The results showed that，the total N2O emission showed a trend of yellow brown paddy soil of Hubei > purple soil of Sichuan > blue mud paddy soil of Zhejiang on the whole.Without inhibitors，PASP-urea significantly reduced the soil N2O emission in yellow brown paddy soil of Hubei and blue mud paddy soil of Zhejiang compared with ordinary urea.When the soil was mixed with DMPP，the N2O emission had a certain decline，with 70%～86% in purple soil of Sichuan，7%～53% in yellow brown paddy soil of Hubei and more than 96% in blue mud paddy soil of Zhejiang compared with no inhibitors added.When NBPT was added，the N2O emission decreased by 14%～22% in purple soil of Sichuan and 26%～60% in blue mud paddy soil of Zhejiang.Therefor DMPP can effectively reduce N2O emission from the tested soil；However，NBPT only reduced N2O emission in Sichuan and Zhejiang tested，soil.In conclusion，DMPP can be applied in purple soil of Sichuan and blue mud paddy soil of Zhejiang，and DMPP，or DMPP combined with NBPT can be used in yellow brown paddy soil of Hubei to reduce N2O emission and its potential harm.The present study provides reference for further research on the regulation mechanism of nitrogen transformation in various regions of Yangtze River basin.